Electrofusion・Oocyte Activation unit CFB16-HB

 

 

 CFB16-HB:  Electrofusion and Oocyte Activation Unit with Genome Editing Function

 

Feature

・ Oocyte activation power module is separated: Appripriate low voltage control
・ The function for zygote genome editing is equiped.
・ Easy-to-use for operation by touch panel
・ All the data of the executed and programmed parameters is exported through USB memory
・ Values of the executed DC pulses are displayed and saved in the unit.


Cat#: CFB16-HB
Product Name: Electrocell fusion/Nuclear Transfer/Genome Editing Machine CFB16-HB
Developed and manufactured by BEX Co., Ltd.
 

  

About CFB16-HB

CFB16-HB is a successor model to LF101 and LF301. CFB16-HB separates oocyte activation power module from cell fusion power module and add the function of zygote genome editing mode. CFB16-HB equips all the functions for the experiments about early embryos. No need to prepare each machine for each experiment for eary embryo.

 

First electrofusion unit with the function of zygote genome editing

CFB16-HB equips the function of "GenomeEdit" mode for zygote genome editing of electroporation and CRISPR-Cas9 system. As well, oocyte activation mode and tetraploid formation mode are available in CFB16-HB. This is the one, CFB16-HB, suitable for all the experiments with early embryos.

  

 

Electrofusion power module and oocyte activation power module are separeted.

Electrical conditions for Body cell fusion (i.e., preparation of hybridoma) and oocyte activation (i.e., preparation of cell body clone) seem similar in view of use of both AC and DC pulses. However, the best parameters such pulse voltage, pulse length, etc. are quite different respectively. In previous versions, a machine need to be coodinated to either mode before shipment for each customer's use. CFB16-HB separated a power unit for body cell fusion from that for oocyte activation. Just 1 press of the button enables to select either mode. 

 

 

All the pulse output data exported to USB memory

CFB16-HB can export all the executed pulse output data to USB memory.

This function saves time to mannually record the output data and executed values in the notebook. 

All the data is exported to USB memory as CSV file

The CSV file is read by Spredsheet software. 

 

All the executed values of output pulses are automatically saved in CFB16-16.

CFB16-HB automatically saves all the data of the executed pulse voltage and current to track the experiment easily. The mannual recording of each of the executed pulse voltage and current value is not necessary. As well, you can output the same parameters as the tracked values in the display

 

"HISTORY" screen can track the parameters. From this screen, the same parameters can be output.

 

Compact body with mutiple functions and easy-to-use touch panel

CFB16-HB equips 5.6 inch touch panel for easy-to-use operation. CFB16-HB is much more compact than the previous version. CFB16-HB is table-top.

 

CFB16-HB Dimensions（Unit is mm）

 

 ※ The spec and appearance of the product(s) above can be changed for improvement without notice.

 

Specification

Fusion Mode

AC Pulse

Voltage		0 - 100 V （1 V resolution）
Output time	Pre-fusion	0 - 120 sec（1 sec resolution）
	Post-fusion	0 - 10 sec（1 sec resolution）

DC Pulse

Wave shape	Square
Voltage	1 - 1500 V （1 V resolution）
Pulse length	3 - 100 μ sec（1 μ sec resolution）
Pulse interval	0.1 - 10 sec （0.1 sec resolution）
Output mode	single polar pulse / bi-polar pulse
Pulse number	Max 100 pulses
Impedance measurement range	Max 40 kΩ

 

 

Activationon Mode

AC Pulse

Voltage	0 - 10.0 V （0.1 V resolution）
Output time	0 - 120 sec （1 sec resolution）
Post-fusion	0 - 10 sec（1 sec resolution）

DC Pulse

Wave shape	Square
Voltage	1 - 200 V （1 V resolution）
Pulse length	3 - 100 μ sec（1 μ sec resolution）
Pulse interval	0.1 - 10 sec（0.1 sec resolution）
Output mode	single polar pulse / bi-polar pulse
Pulse number	Max 100 pulses
Impedance measurement range	Max 40 kΩ

 

 

GenomeEdit Mode

Voltage	1 - 200 V　(1 V resolution）
Max output current	1 A (1000 mA)
Pulse length（Pon)	0.1 - 1000 ms
Pulse interval	1 - 1000 ms
Pulse number	1 - 1000 pulses (for single polar Pd(+)） 1 - 500 pulses (for bi-polar of Pd(+/-) or Pd(Alt))
Impedance measurement range	Max 4 kΩ

 

Miscellaneous

Size	W 240 mm X L 380 mm X H 190 mm (w/o rubber stand, umbo)
Weight	12 kg
Power	100 - 115 or 220 V 50/60 Hz
Power consumption	260 VA

  

 

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designing and developing