LF101 Cell Fusion Unit

LF101（Cell Fusion, Nuclear Fusion, Oocyte Activation）

◆Application◆

●Cell fusion
Mammarian cells Hybridoma Preparation
Plant cells Protoplast
Yeast
 

●Somatic nuclear transfer
Clone of Bovine・Pig・Cat・Dog・Wolf
ES Cell Preparation by Nuclear Transfer
 

●Oocyte activation
Electrical activation of fore- or post- ICSI（intracytoplasmic sperm injection）

 

Feature

LF101 fits many applications

LF101 can generate ~930V DC pulse, and thus fits many applications from plant cell fusion to mammalian cell fusion. The nuclear transfer model focuses on the accuracy of the voltage generally used for the research (around 50V). LF101 can generate such an accurate square pulse that LF101 can lead high fusion efficiency and viability.

Flexible setting of AC

You can flexibly set AC for both pre-fusion, which makes the pearl chains of the cells, and post-fusion, which improves the cell maturity.

 

Manual Generation

You can set and memorize the program to LF301 for the daily experiment to free you from every day setting. Moreover, DC pulse can be generated manually. Use the manual mode to search the electrical condition.

 

Easy-to-Use with Foot Switch

Various electrodes fit a lot of applications.

We have a collection of the electrode chambers for both large and small batches. Moreover, we provide the acrylic box to avoid contamination. For the nuclear transfer, we developed many needle electrodes. The chamber electrodes limit the number of operation at once. Instead, nuclear transfer with the needle electrodes is effective and saves time because the oocytes to be operated are placed in the dish. The fusion is more efficient as the quality of the oocytes is  confirmed by eyes and bad oocytes are removed beforehand. We have the dish electrodes to activate oocytes.

Specification for AC

AC wave	sine wave
AC voltage	0-50Vp-p
Fluency	1MHz
Time	0.1-3.9MHz
post-Fusion	Available （On or Off）
Connected load	< 50Ω

Specification for DC pulse

DC pulse	Square
DC voltage	0-930V, 0-200V(by the 1V) *
pulse time	5-99μs(by the 1μsec)
pulse inteval time	0.1-9.9s(by the 0.1sec)
pulse number	0-100 time(s) (automatic or manual)

* The special edition for cloning the animal generates 0-200V

Specification

 

Impedance measurement	0-39.99 kΩ
AC/DC changing time	~ 5μs
Memory track	99 tracks
Power	100－240V （free）
Size	375W×360D×170Hmm

 

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