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Electroporator for Zygote Genome Editing

Electroporator for Zygote Genome Editing
Genome Editor^TM

Genome Editor

Genome Editor^TM is a simple and easy-to-use electroporator suitable for zygote genome editing using CRISPR/Cas9 system.

Cat #: GEB15
Product Name： Genome Editor
Made by: BEX CO.,LTD.

Application

High throughput and effcient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote

Fgf10 gene knockout mouse by Cas9 mRNA/gRNA electroporation

Fgf10 homozygous mutant embryo have an easily detectable limbless phonotype. Electroporation of Cas9 mRNA (Cas9 Protein) and gRNA enables genome editing of Fgf10.

Electrodes for zygote genome editing

Platinum plate electrode on tempered glass, LF501PT1-10, can hold up to ~40 mouse zygotes in a 5 μL electroporation buffer and enables efficient and high throughput zygote genome editing using CRISPR/Cas9 by electroporation.

Electroporation vs Microinjection

	Electroporation	Microinjection
Pre-operation	Not necessary	Preparation of injection and hold pippettes, etc.
Required time for 100 zygotes	~5 minutes	> 2 hours
Viability (after E15)	40-80%	10-50%
Efficiency	Same as microinjection	Depends on the sequence
Cost for devices	About $12,000	> $50,000
Required skills	Not necessary	Maniipularion of single zygotes
Required amount of Cas9 mRNA	500 - 2,000 ng	50 - 500 ng

More zygotes can be treated at one time by electroporation than microinjection.

Viability of treated zygotes are much higher in electroporation than in microinjection.

No special skills are required for electroporation compared to microinjection, which requires injection technique that is time-consuming to acquire.

Electroporation is , therefore, the ideal method for high throughput mouse zygote genome editing by CRISPR/Cas9 system.

References

1. Hashimoto M, Takemoto T. Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Sci. Rep. 2015; 5: 11315.

2. Hashimoto M, Yamashita Y, Takemoto T. Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Dev. Biol. 2016; 418(1); 1-9.

3. Tanihara F, Takemoto T, Kitagawa E, Rao S, Do LTK, Ohnishi A, et al. Somatic cell rprogramming-free generation of genetically modified pigs. Sci. Adv. 2016; 9(2); e1600803.

Specifications

Voltage	1 - 200 V / 1 V resolution
Max current	1000 mA
Pulse length （Pon)	0.1 - 1000 ms
Pulse interval (Poff)	1.00 - 1000 ms
Pulse number	1 - 1000 for Pd(+) 1 - 500 for Pd(+/-) and Pd(ALT)
Impedance measurement	Up to 4.00 kΩ
Voltage measurement	-200 V - +200 V / 1 V resolution
Current measurement	-1023 mA - +1024 mA / 1 mA resolution
Memorable programs	>20000 programs
History	Last 100 histories are saved. History data can be exported to a USB memory in CSV format.
Power	100 - 115 or 220 V、50/60 Hz
Fuse	10 A (6.3 mm x 20 mm)
Dimensions	W 240 mm X L 380 mm X H 190 mm (w/o rubber stand, umbo)
Weight	5.5 kg