BEX CO., LTD.
DNA-Free Genome Editing
Electroporator for Plant
Genome EditorTM for Plants NEW
3rd version of Genome Editor series！ An Electroporator to perform DNA-Free genome editing
Cat#：GEBPL
Product Name： Genome Editor for Plants
Manufactured by：BEX CO.,LTD.
New technology of innovative electrical pulse, developed by BEX achieves highly efficient delivery of Cas9 to protoplasts.
New and innovative technology to make an instant change from + charge to - achieves more efficient delivery than ordinary electroporation.
We succeeded in knock-in for plants as well as knock-out.
Genome Editor for plants is operated by 5.7 inch touch panel. It is very easy to operate Genome Editor for plants visually by the touch panel. The visual operation avoids the mistakes of which parameter to be set.
Genome Editor for plants is desktop-size, easy-to-move.
Genome Editor for plants automatically records the last 100 runs of data of executed volatege and current. It is not necessary to make a note of values of each electroporation. This function makes the experiments more efficient. Genome Editor for plants displays the executed parameter and can generate the same parameter as the displayed parameter.
Comparison between previous pulse pattern and fall pulse pattern for tobacco protoplast electroporation
Fall Pulse. Fall pulse perfroms highly efficient delivery of GFP plasmid DNA.
Auto-fluorescene specific in plants is note detected as PDS gene is knockout by design of gRNA on phytoene desaturase gene of Carotenoids biosynthesis
In comparison with control, green color on the plant disappears in the bright field as well as auto-fluorescene.
dsDNA with GFP cassette is electroporated with gRNA and Cas9 to tobacco cells (protoplasts). After regeneration, GFP fluorecene is detected.
* Tobacco cells above were prepared from the tobacco benthamiana leaf.
Constant voltage pulse【Square(V)】mode
Poratio （PLPp）
| Voltage | 1 - 350 V (1 V resolution） |
|---|---|
| Max current | 10 A |
| Pulse pattern | Options: PLPp(+)：single polar |
| Pulse number | 1 -10 （ON/OFF switching available） |
| Decay rate | 0 - 50% in 10% resolution |
| Pulse length （Pon) | 0.05 - 1000 ms |
| Pulse interval（Ppoff)*1 | 50.0 - 1000 ms |
| Pulse interval（Ppoff2)*1 | 0.0 - 1000 ms |
| Condenser capacity | PLPp (+): 2,429 uF PLPp (+/-), PLPp (Alt): 1,416 uF |
*1 In PLPp(ALT) mode, Pp off2 is the interval between a + polar pulse and a －polar pulse, and Pp off1 is the interval between sets. (A set contents of a + polar pulse and a －polar pulse .
Driving pulse(Pd)
| Voltage | 1 - 200 V 1 - 250 V in 1 V resolution） |
|---|---|
| Maximum current | 1 A (1,000 mA） |
| Pulse length (Pdon) | 0.05 - 1000 ms Resolution - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms |
| Pulse interval*2 (Pdoff) | 0.05 - 1000 ms |
| Decay rate | 0 - 100% in 1% resolution |
| Number of Pulse | 1 - 1,000 cycle in PLPp(+) 1 - 500 cycle in PLPp(+/-) or PLPp(ALT) Pd output is switchable: ON/OFF |
*2 When Decay rate is over 0 %, the minimum interval is 0.1 ms
| Pulse shape | Decay pulse |
|---|---|
| Voltage | 1 - 400 V in 1 V resolution |
| Maximum current | 10 A when Pd is decay pulse or 2 A when Pd is square pulse |
| Pulse length (Pdon) | 0.01 - 99.9 ms Resolution - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms |
| Pulse interval (Pdoff) *3 | 0.05 - 99.9 ms Resolution - 0.01 ms between 0.05 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms |
| Number of Pulse | 1 （Pp output is switchable: ON/OFF） |
*3 The interval between poration pulse (Pp) and driving pulse (Pd)
Decay pulse【Decay(V)】mode
| Voltage | 1 - 350 V in 1 V resolution |
|---|---|
| Maximum current | 10 A |
| Pulse length（Pdon) | 0.05 - 1000 ms Resolution - 0.01 ms between 0.05 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1000 ms |
| Pulse interval (Pdoff) | 0.05 - 1000 ms Resolution - 0.01 ms between 0.05 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1000 ms |
| Condenser capacity | Choice from 3.3 uF, 10 uF, 33 uF, 100 uF, 330 uF and/or 940 uF |
| Number of pulse | 1 -1,000 cycle（Pp output is switchable: ON/OFF） |
Constant voltage square pulse【Square(V)】mode
| Voltage | 1 - 200 V in 1 V resolution |
|---|---|
| Maximum current | 1 A (1,000 mA） |
| Pulse length（Pdon) | 0.05 - 1000 ms Resolution - 0.01 ms between 0.05 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1000 ms |
| Pulse interval*4 | 0.05 - 1000 ms Resolution - 0.01 ms between 0.05 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1000 ms |
| Decay rate | 0 - 99 % in 1 % resolution |
| Number of pulse | 1 -1,000 cycle (Pd output is switchable: ON/OFF） |
*4 When Decay rate is over 0 %, the minimum interval is 0.1 ms
Miscellaneous
| Impedance measurement | 0 - 39 kΩ Resolution - 0.001 kΩ between 0 - 0.999 kΩ - 0.01 kΩ between 1.00 - 9.99 kΩ - 0.1 kΩ between 10.0 - 19.9 kΩ - 1 kΩ between 20 - 39 kΩ |
|---|---|
| Voltage measurement | -512 V － +511 V in 1 V resolution |
| Current measurement | -10.23 A － +10.24 A in 0.01 A resolution when Pd is decaying -1,023 mA － +1,024 mA in 1 mA resolution when Pd is square |
| Memory | Over 20,000 programs are recordable |
| History record | The last 100 programs are saved. These programs are exported to a PC by USB device. CSV format file(.csv) is exported. |
| Power | single phase 100 - 115 or 220 V 50/60 Hz |
| Fuse | 10 A（6.3 mm X 20 mm） |
| Dimensions・Weight | (W) 360 mm × (L) 380 mm × (H) 180 mm, 9.0 kg |
We welcome the request of maintenance support for electroporators (CUY21 series: CUY21EDIT II, CUY21EDIT, CUY21Vivo-SQ and CUY21Vitro-EX), cell fusion units (LF101 and LF301), electrodes, other accessories. Our engineers will be in direct charge of the products. Please ask us.
CUY21Vivo-SQ(In vivo electroporator)
CUY21EDIT（In vivo electroporator）
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