Monoclonal Antibody Preparation

Detail

Quality-guaranteed Monoclonal Antibody Preparation

・In the system of “Quality-Guaranteed” the customers are free from the risk unique to the biotechnological sample preparation: Even if the preparation is failed, the money will never come again. Therefore, we provide Quality-Guaranteed service of monoclonal antibody preparation. If the clone providing antibodies isn’t obtained, the fee isn’t payable. The condition of the “free” is that no positive well is observed in the ELISA screening.

Quality・Price・Delivery

Keeping mice in good condition is the key to prepare a good antibody We apply the method to lose the damage to mice in the immunization operation and make two immunizations and final booster. Moreover, the systemized schedule from immunization to establishing hybridoma can give you both of the low price and accurate delivery. 

Price and Delivery

Monoclonal Antibody Preparation

	Price	Delivery
Mainly mice: immunization to hybridoma establishment Max 10 clones	¥900,000 / 1 sample	About 3 month ～

 Option

	Price	Delivery
Affinity Purification （ < 10ml ）	¥120,000 / 1 sample	About 2 weeks ～
Protein A Purification（ < 10ml）	¥85,000 / 1 sampke	About 2 weeks～
Ascites Preparation	￥20,000 / 1 animal	About 1 month ～
Additional Clone	￥35,000 / one clone	Please ask us

Flow of Operation

1,Antigen
Please get over 1mg of antigen ready
A small amount is available.

2,Mouse
Balb/c for mice or Wister for rats are used in case of no request about the species.

3,Immunization
Two immunizations and final booster to the mouse.
First selection of Titer-MAX in the case of protein antigen.
Adjuvant may not be used in the case of bacteria and cells.

4,Cell fusion
Cell fusion by PEG method with mouse myeloma P3U1 is carried out indivisually.
Lymphocyte cells are obtained from the spleen and lymph node.
PEG1500 is used for cell fusion.

5,Screening
ELISA is used for the antibody screening. The plate combined with bacteria and/or cells is acceptable. The antibody to immunized antigen is selected.


～Free if Step 4 isn't reached for the 'Quality-Guaranteed monoclonal preparation.～

6,Sample delivery
The supernatants of the selected clones cultured in the 24-well plate is delivered. Please evaluate the samples.

7,Cloning
Selected clones are cloned (Max 10 clones)
Two clonings are usually conducted by limiting dilution method.
The preparation of cloning does NOT include 'Quality-Guaranteed' service because some of the clones might lose the ability to produce antibodies.

8,Clone establishment and frozen ampule preparation
Established hybridoma are proliferated. > 3 frozen tubes for each clone are prepared. Subclass will be measured by the kit.

Delivery products are

・>= 3 frozen hybridoma vials for one clone (3~5 X 10^6 cells /vial)
・Cultured supernant
・Subclass data

 

Flow to Order

1.Please send the order form to us by email.
 

2.Please send the sample and document described below to us,
 

・Order form
・Antigen ※Please keep frozen during the delivery
The shipment is not necessary if BEX prepares antigen.

Address to ship the sample and document to

BEX Co., Ltd.
4th Floor, 2-61-15 Itabashi, Itabashiku-ku Tokyo 173-0004 Japan
TEL. 03-5375-1071

Delivered Product

Delivered Product

・>= 3 frozen hybridoma vials for one clone (3~5 X 10^6 cells /vial)
・Cultured supernant
・Subclass data
・The rest of antigen

 

Attention

・The quality-guaranteed may not be acceptable if the preparation is extremely difficult, 
・If the preparation is not applied to the quality-guaranteed, the actual fee shoud be payable in the unavoidble termination during the operation.
・・The delivery date will be changeable according to the operation,
・The freight of the sample to BEX should be payable.

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