Protein Expression by Mammalian Cells

Detail

・Transfection to Mammalian cells, and preparation and purification of the targeted protein.
・HEK-293, COS-7, and CHO are usually used. The other cells or the cells the customer owns are available.
・Amino acid substitution, defective mutant, etc are acceptable to establish the expression system.
・Please ask us about expression volume if a big volume is required.

Price and Delivery

	Price	Delivery
Construction of the vector※1	￥80,000 / 1 sample	2 weeks～
Expression Analysis※2	￥150,000 / 1 sample	1 month ～
Small-Amounted Culture（100mL)・Purification Review※3	￥200,000 / 1 sample	1 month ～
Large-Amounted Culture (1L) ・Purification※4	￥400,000 / 1 sample	2 month ～

※1 The vector to add His-Tag or GST-Tag is used. Any other tag is cceptable. Please ask.
The price and delivery date to construct the Expressing vector depends on the operation. Please refer to subcloning' and/or cDNA cloning'.”発現ベクター構築料金は、作業内容により変動いたします。詳細は弊社受託サービスの'および'をご参照ください。
※2 SDS-PAGE and Western Blotting is used to check the expression after small scaled transfection.
※3 The review may cause no acceptability for the large-amounted culture and purification afterwards.
※4 Affinity purification is used. Dialysis and/or gel filtration are acceptable (resulting in additional fees). The condition for the expression should be provided in advance in the case of the order only of large-amounted culture and purification,

Flow of Operation

1,Establish the Vector
Preparation of the targeted gene from your vector and/or PCR  production.  
Check of insertion sequence.

2,,Expression Analysis
The vecotr is transfected to the targeted cell
SDS-PAGE and Western Blotting is used to check the expression.

3,,Small-Amounted Culture・Purification Review
Yield and purity check after expression and purification (100mL)

4,Large-Amounted Culture・Purification
Large-amounted culture under the condition decided above.
Purification by Tag-specific affinity columns
 

Flow to Order

1.Please send the order form to us by email.

2.Please send the sample and document described below to us: 

・Order form 
・Vector 2μg( > 100ng/μl） / cDNA（ > 1μg) / Cell （5×106 cells） /     ベクター 2μg(100ng/μl以上） / Tissue （about 10mg） / Total RNA（ > 10μg） / etc. 
※Please keep the sample frozen during the delivery.

Address to ship the sample and document to

BEX Co., Ltd.
4th Floor, 2-61-15 Itabashi, Itabashiku-ku Tokyo 173-0004 Japan
TEL. 03-5375-1071

Delivered Products

Delivered Products

Purified Protein
Data of Expression Analysis
Operation Reports

Attention

・The yield depends on the expressed protein.
・If necessary, NDA is agreeable. The operation is conducted under the NDA.
・The actual fee is payable in the unavoidable termination after the discussion with the customers.  
・The delivery date depends on the operation. 
・The freight of the sample to BEX is payable.

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