DNA Sequencing

Detail

・Next-day reports of the sequence data and spectra data (PDF or AB1 formatted) by email.
・Strict control for the customer's sample information by well-trained staff.
・Dye terminator method with 4 color fluorescenes is used for analysis.

Price・Delivery

DNA Sequencing

	Price	Avg. Analyzed Length	Delivery
Reaction＋Analysis※1	￥3,500 / 1run	600bp～800bp	1 business day～
preMix※2	￥12,800 / 8run (￥1,600/ 1run)	600bp～800bp	1 business day～
Analysis※3	￥1,000 / 1ruｎ	600bp～800bp	1 business day～
Analysis※3　plate( >= 96run)	￥500 / 1run	600bp～800bp	4 business day～

※1　The fee turns to be ¥2,500 / run in the case that the number of unanalyzed bases is more than that of BEX QC Standard bases.  
※2　Please send 8-strip tube with the mix of templates and primers. Even if the number of the samples are not up to the eight units, the fee for 8-run units (¥1.600/run) should be payable.  
※3　The samples reacted to ABI BigDye® v1.1 or v3.1 are acceptable in the service of Analysis. The samples should be reacted, purified, and dried up.  

Option

	Price
PCR Product Purification （Single Bands Only Acceptable）	￥1,000 / sample
Email with Spectra Data PDF File and AB1 File	￥1,000 / order
CD-R Shipment with Spectra Data PDF File and AB1 File	￥2,000 / order
Shipment of the Document of Spectra Data	￥1,000 / order

How to Order

1.Please send the order form to us by email.

2.Please send the sample and document described below to us:

 

Reaction＋Analysis
・Order form
・For Purified Plasmid DNA
Total < 7kbp・・・ > 700ng
Total > 7kbp・・・ > 1μg
（ > 50ng/μL）※Please keep the sample chilled during delivery.
・PCR product
Purified・・・ > 100ng
Unpurified・・・PCR Solution 50μL（* Additional fee for unpurified）※Please keep the sample chilled during delivery.
・Primer※4
> 10pmol / run （10μM）※Please keep the sample chilled during delivery.

※4　Universal Primer is Free in Reaction ＋ Analysis

Primer Name	Sequence
M13 F-1	GTAAAACGACGGCCAG
M13 F-2	GTTTTCCCAGTCACGAC
M13 R	GGAAACAGCTATGACCATG
T7	AATACGACTCACTATAGG
T3	AATTAACCCTCACTAAAGG
SP6	ATTTAGGTGACACTATAG
10F	GTTTGATCCTGGCTCA
800R	TACCAGGGTATCTAATCC
ITS1F	GTAACAAGGT(T/C)TCCGT
ITS1R	CGTTCTTCATCGATG

 

preMix
・Order form
・ 8-strip tube units with the mix of the template and primers

Please prepare the samples per 1 run like below:
-PCR Product: （Please purify the PCR product after checking single bands）
100-200bp → 1-3ng
200-500bp → 3-10ng
500-1000bp → 5-20ng
1000-2000bp → 10-40ng
2000bp以上 → 20-50ng
-Purified Plasmid DNA: 
Total < 7kbp・・・150-300ng
Total > 7kbp・・・300-500ng 
-Primer:
3.2pmol
※Total solution should be 14μL / run in DDW.
※The sample numbers are labeled in the 8-strip tube.
※An additional fee is ¥1,000 / tube for the universal primer.

Address to ship the sample and document to

BEX Co., Ltd.
4th Floor, 2-61-15 Itabashi, Itabashiku-ku Tokyo 173-0004 Japan
TEL. 03-5375-1071

Delivered Product

By e-mail

・Sequence Data （Text File） 
・Sequence Data （AB1 formatted）
Either PDF or AB1 formatted

Attention

・Next-day reports require the arrival of the samples by noon. (Except Saturday, Sunday, Holidays) 
・Free reanalysis is conducted in the conditions listed below in DNA Sequencing service: 
1) Analysis expected in another condition , The spectra data are unclear because the analysis equipment doesn’t work. However, 1) condition requires the order including the service of “Reaction ＋ Analysis” 
・The sample is adequately discarded under the strict security, law, regulation, and rules.
・The freight of the sample to BEX is payable.

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