Next-Generation Electroporator CUY21EDITⅡ

 

 
Next-Generation Electroporator

 

 CUY21EDIT II

 

NEW

 

・ The genuine successor for CUY21EDIT, which is the best in vivo electroporator 

・ CUY21EDIT II generates the accurate squere pulse for in vivo and decaying pulse for in vitro

・ CUY21EDIT II is the true electroporator, which two power units suitable for in vivo and in vitro respectively

・ CUY21EDIT II is the first electroporator to generates constant current pulses.

・ Constant current pulses is the best for GONAD as well as in vivo electroporation.

・ CUY21EDIT II is half as heavy as CUY21EDIT

・ Easy to use with 5.7 inch touch panel.

・All data of executed voltage and current of generated pulses are recorded.

・ The pulse records is exported to a USB memory


Catalog Number: CUY21EDIT2
Product Name: in vivo and in vitro electroporator CUY21EDIT II
Made by BEX CO.,LTD.

CUY21EDIT II electroporates a lot of samples such as tissues, adhesive cells, cell lines, primary cells with various unique electrode

・in vivo electroporation
  (in utero(exo utero)electroporation,
   in ovo electroporation)
・ex ovo electroporation
・ex vivo electroporation
・in vitro electroporation
・adhesive cells electroporation
 

 

 

  

CUY21EDITⅡ(in vivo&in vitro electroporator)

CUY21EDIT II is the true succesor of the best electroporator CUY21EDIT, which has a lot of great performance. CUY21EDIT II is easy to be used and generates the accurate pulses. As well as succeeding the great function of CUY21EDIT, BEX technology enable CUY21EDIT II to generate every pattern of the pulses for both of in vivo and in vitro. Moreover, CUY21EDIT II is the first electroporator to generate constant current pulses. CUY21EDIT II performs combination of constant voltage pulses and constant current voltage pulses, which leads to a whole new electroporation world !

Every pattern of pulses

CUY21EDITⅡ（in vivo&in vitro electroporator） generates decaying pulses as well as square pulses CUY21EDIT generates. Moreover, CUY21EDIT II generates dual pulses CUY21EX generates. That is, CUY21EDIT II also generates 1. Poration Pulses (Pp), which make very  small holes in the surface of the cell, and 2. Driving Pulses (Pd), which move DNA, RNA, etc. into the inside of the cell. CUY21EDIT II changes the polarity of  Pd in every way.

 

 

 

 

 

 

 

 

5.7-inch touch panel for easy use

CUY21EDIT II is used by 5.7 inch touch panel. It is very easy to operate CUY21EDIT II visually by the  touch panel. The visual operation avoids the mistakes of which parameter to be set. 

 

 

The first electroporator to generate constant current pulses

CUY21EDIT II is the first electroporator to generate constant current pulses. In electroporation, the key to success is current which is running between the sample. Previous electroporators, however, set only voltage value and can't set current running between the sample. BEX previously equipped all BEX electroporators with the function to measure the impedance, so that the user can calculate the current by the set voltage and measured impedance. Now, CUY21EDIT II initialy can set current values to release the user from the calculation of the current value by the voltage and the impedance.

 

 

Pulse data is exported to a USB memory.

CUY21EDIT II exports all data of generated pulses to a USB memory. It is not necessary to make a note for electroporation data. You can use digital data in your PC.

 

 

A lot of application

CUY21EDIT II generates every pattern of the pulses to fit every application of electroporation. As well as in vivo electroporation,  CUY21EDIT II is suitable for electroporation for suspension cells of animals or plant cells and for electroporation with adhesive cells attached in the plate or dish. CUY21EDIT II is used for iPS cell research.

 

 

All data of executed voltage and current pulses are recorded.

CUY21EDIT II records all data of executed volatege and current. It is not necessary to make a note of values of each electroporation. This function makes the experiments more efficient. CUY21EDIT II displays the executed parameter and can generate the same parameter as the displayed parameter.

 

'History' display to track the parameter. CUY21EDIT II can generate this parameter in this display.

 

※The spec and appearance of the product(s) above can be changed for improvement without notice.

 

 

Applications

GONAD

GONAD: Genome editing via Oviductal Nucleic Acids Delivery
is a simple and powerful method to genome edit zygotes by electroporation of the oviduct with zygotes inside.

 

Easy 3 Steps！

 

 

 

 

 

 

 

 

 

 

 

That's it！！

 

 

Result

２Result of 2-cell embryos after electroporation

 

Advantage of Constant Current for GONAD

 

Ex: When the oviduct is grabbed by the tweezer-like electrode...

Even if voltage is constant, current value depends on the distance between the electrodes, the condition (oxidization) of the electrodes, etc.

 

In constant current mode (Square (mA) mode), CUY21EDIT II controls as current runs constantly.

 

That is, in GONAD with CUY21EDIT II and tweezer-like electrodes (LF650P series), constant current runs through the oviduct even if  the strength to grab the oviduct is different in each trial. 

 

The electrical parameter is fixed in in vivo electroporation, which has many parameters for optimization.

 

 

Zygote Electroporation (GEEP method)

Please visit herefor GEEP

 

 

 

Spec

Poration Pulse (Pp)

Voltage	1 - 400 V in 1 V resolution
Pulse length (Ppon)	0.01 - 99.9 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms
Pulse interval* (Ppoff)	0.01 - 99.9 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms
No of pulses	1

*Interval between poration and driving pulse

Driving Pulse (Pd)

Decay (V) mode (Decay pulse)
Voltage	1 - 350 V in 1 V resolution
Maximum current	10 A
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Condenser capacity	Choice from 3.3 uF, 10 uF, 33 uF, 100 uF, 330 uF and/or 940 uF Multiple choices are avialable, Min: 3.3 uF; Max: 1,416.3 uF
No of pulses	1 - 1,000 number(s); On-Off switchable

 

Driving pulse (Pd)

Square (V) mode (Constant-voltage square pulse)
Voltage	1 - 250 V in 1 V resolution
Maximum current	1 A (1,000 mA)
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval** (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 99% in 1% resolution.
No of pulses	1 - 1,000 number(s); On-Off switchable

** In case decay rate is not 0%, the minimum time is 0.1 ms.

 

Driving pulse (Pd)

Square (mA) mode (Constant-current square pulse)
Current	1 - 1,000 mA in 1 mA resolution
Maximum voltage	200 V
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval** (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 99% in 1% resolution.
No of pulses	1 - 1,000 number(s); On-Off switchable

** In case decay rate is not 0%, the minimum time is 0.1 ms.

Decay (V) + Square (V) mode

Plural poration pulse (PLPp)
Voltage	1 - 350 V in 1 V resolution
Maximum current	10 A
Pulse length (PLPpon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval (PLPpoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 50% in 10% resolution.
Condenser capacity	PLPp (+): 2,429 uF PLPp (+/-), PLPp (Alt): 1,416 uF
No of pulses	1 - 1,000 number(s); On-Off switchable

 

Driving poration pulse (Pd)
Voltage	1 - 200 V in 1 V resolution
Maximum current	1 A (1,000 mA)
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 99% in 1% resolution.
No of pulses	1 - 1,000 number(s); On-Off switchable

Miscellaneous

Impedance measurement	0 - 39KΩ Resolution: - 0.001KΩ between 0- 1KΩ - 0.01KΩ between 1 - 10KΩ - 0.1KΩ between 20 - 39KΩ
Voltage measurement	-512 - +512V in 1V resolution
Current measurement	-10.23A - +10.24A in 0.01A resolution (Pd is decaying) -1023mA - +10.24mA in 1mA resolution (Pd is square)
Memory	4GB (Over 20000 programs are recordable)
History record	The last 100 programs are saved. These program are exported to a PC by USB device. CSV format file(.csv) is output.

 

Other

Power	100 - 115 or 220 V 50/60 Hz
Fuse	10 A (6.3 mm X 20 mm)
Dimensions	W 240 mm X L 400 mm X H 203 mm
Weight	9 kg

in vivo&in vitro electroporation Application

A lot of applications for electroporation to tissues, adhesive cells, etc. with CUY21 series by BEX (electroprator) and a lot of various unique electrodes.

Electrode for CUY21 series
 
Reference for CUY21 series (electroporation)

 

method	species	target
in vivo　electroporation （CUY21）	adult mouse	skin, musle, kidney, liver, spleen, blood tube, bladder, testis, ovary, brain, brown adipose tissue, zygote
	adult rat	muscle
	new born mouse and rat	brain, retina, cornea
	hamster	muscle
	rabbit	muscle, knee joint
	zebrafish	tail fin, retina
	Xenopus	embryo, retina (tadpole)
	cricket	embryo
	honey bee	brain
	Terrestrial slug (Limax valentianus)	Whole body
in utero electroporation （CUY21）	mouse embryo	brain, spinal cord, retina, internal ear
	rat embryo	brain
ex utero electroporation （CUY21）	mouse embryo	brain
	mouse / rat embryo	whole embryo (brain slice, neural tube)
in ovo electroporation （CUY21）	chick embryo	neural tube, eye capsule, leg bud (mesoblast), body segment (mesoblast),  plan heart area (mesoblast), posterior mesoblast, plan trache-bronchi area, blastodermic layers
	uzura embryo	neural tube
ex ovo electroporation （CUY21）	chick early embryo	New’s culture
	chick embryo	liver, cerebellum
ex vivo electroporation （CUY21）	mouse	brain, retina, small intestine, tooth germ, internal ear
	rat	brain
	chick embryo	spinal cord, brain stem, retina, anterior stomach
in vitro electroporation （CUY21）	adhensive
	primary cells , cell lines, etc.

 

Maintenance support for electroporators, cell fusion units, electrodes.

We welcome the request of maintenance support for electroporators (CUY21 series: CUY21EDIT II, CUY21EDIT, CUY21Vivo-SQ and CUY21Vitro-EX), cell fusion units (LF101 and LF301), electrodes, other accessories. Our engineers will be in direct charge of the products. Please ask us.

Lifescience instrument

in vivo & in vitro electroporator

in vivo electroporator

in vitro electroporator

Cell fusion unit

Cables and Accessaries

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micromanipulator

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