StraightWalk® kit (RightWalk® kit)

StraightWalk® kit

The adaptor is added to the gene after the restriction enzyme treatment. PCR is conducted with the primer designated for the known sequence. It is possible to subclone to various vectors by adding suitable restriction enzyme sites to known primers because the sequence of the primer includes the cutting site for NotⅠ 
By use of StraightWalk® Kit (previously called RightWalk® Kit), An unknown region is cloned from the sample ten times as a large genome size as human.

 

Original article for StraightWalk® method

Tsuchiya T, Kameya N, Nakamura I. Straight Walk: A modified method of ligation-mediated genome walking for plant species with large genomes. Anal. Biochem. 2009; 388: 158-160.

 

References

Kawamura M, Umehara D, Odahara Y, Miyake A, Ngo MH, Ohsato Y, Hisasue M, Nakaya M-a, Watanabe S, Nishigaki K. AKT capture by feline leukemia virus. Arch, Virol. in press

 

Igarashi H, Koizumi K, Kaneko R, Ikeda K, Egawa R, Yanagawa Y, Muramatsu S-i, Onimaru H, Ishizuka T, Yawo H. A novel reporter rat strain that conditionally expresses the bright red fluorescent protein tdTomato. PLoS ONE 2016; 11(5): e0155687.

 

Kawahigashi H, Nonaka E, Mizuno H. Kasuga S, Okuizumi H. Classification of genotypes of leaf phenotype (P/tan) and seed phenotype (Y1 and Tan1) in tan sorghum (Sorghum bicolor). Plant Breed. 2016; 135(6): 683-690. 

 

Koeda S, Sato K, Tanaka Y, Takisawa R, Kitajima A. A Comt1 loss of function mutation is insufficient for loss of pungency in Capsicum. Am. J. Plant Sci. 2015; 6: 1243-1255.

 

Yabuki A, Toyofuku T, Takishita K. Lateral transfer of eukaryotic ribosomal RNA genes: an emerging concern for molecular ecology of microbial eukaryotes. ISME J. 2014; 8: 1544-1547.

 

Ikegami H, Nogata H, Inoue Y, Himeno S, Yakushiji H, Hirata C, Hirashima K, Mori M, Awamura M, Nakahara T. Expression of FcFT1, a FLOWERING LOCUS T-like gene, is regulated by light and associated with inflorescence differentiation in fig (Ficus carica L.). BMC Plant Biol. 2013; 13: 216.

 

Ishikawa G, Seimiya Y, Saito M, Nakamura T, Hoshino T. Molecular characterization of spontaneous and induced mutations in the three homologous waxy gene of Japanese barnyard millet [Echinochloa esculenta (A. Baun) H. Scholz] Mol. Breed. 2013; 31(1): 69-78.

 

Nakamura K, Akiyama H, Kawano N, Kobayashi T, Yashimatsu K, Mano J, Kitta K, Ohmori K, Noguchi A, Kondo K, Teshima R. Evaluation of real-time PCR detection methods for detecting rice products contaminated by rice genetically modified with a CpTI-KDEL-T-nos transgenic construct. Food Chem. 2013; 141(3): 2618-2524.

 

Suzuki A, Ishida Y, Aizawa S, Sakata I, Tsutsui C, Mondal A, Koike K, Sakai T. Molecular identification of GHS-R and GPR38 in Suncus murinus. Peptides 2012; 36(1): 29-38.

 

Anai Y, Ochi H, Watanabe S, Nakagawa S, Kawamura M, Gojobori T, Nishigaki K. Infectious endogenous retroviruses in cats and emergence of recombinant viruses. J. Virol. 2012; 86(16): 8634-8644. 

 

Kawabe S, Yokoyama Y. Novel Isoforms of Heat Shock Transcription Factor 1 Are Induced by Hypoxia in the Pacific Oyster Crassostrea gigas. J. Exp. Zool. 2011; 315: 394-407.

 

Yamasaki S, Oda M, Koizumi N, Mitsukuri K, Johkan M, Nakatsuka T, Nishihara M, Mishiba K-i. De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenic gentian.Plant Biotechnology 2011; 28: 223-230.

 

Patent

NEW OLIGONUCLEOTIDE ADAPTOR AND PRIMER　（JP,4603798,B）

 

Mechanism of Straight Walk®

 

 

Contents of Straight Walk® Kit

SWA-1 (Straight Walk Adaptor-1)	For 12 ligations
SWA-2 (Straight Walk Adaptor-2)	For 12 ligations
WP-1 (Walking Primer- 1)	For 48 PCRs
WP-2 (Walking Primer- 2)	For 48 PCRs
λDNA 1 PCR Primer	For 8 trials
λDNA 2 PCR Primer	For 8 trials
dGTP and dCTP	For 12 trials

 

 Price for Straight Walk® Kit

Cat.No.	Product Name	Price
02-0201	Straight Walk® kit	￥25,000

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