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Next-Generation Electroporator CUY21EDITⅡ

Next-Generation Electroporator

CUY21EDIT II

NEW

EDIT2

・ The genuine successor for CUY21EDIT, which is the best in vivo electroporator

・ CUY21EDIT II generates the accurate squere pulse for in vivo and decaying pulse for in vitro

・ CUY21EDIT II is the true electroporator, which two power units suitable for in vivo and in vitro respectively

・ CUY21EDIT II is the first electroporator to generates constant current pulses.

・ Constant current pulses is the best for GONAD as well as in vivo electroporation.

・ CUY21EDIT II is half as heavy as CUY21EDIT

・ Easy to use with 5.7 inch touch panel.

・All data of executed voltage and current of generated pulses are recorded.

・ The pulse records is exported to a USB memory

Catalog Number: CUY21EDIT2
Product Name: in vivo and in vitro electroporator CUY21EDIT II
Made by BEX CO.,LTD.

CUY21EDIT II electroporates a lot of samples such as tissues, adhesive cells, cell lines, primary cells with various unique electrode

・in vivo electroporation
(in utero(exo utero)electroporation,
in ovo electroporation)
・ex ovo electroporation
・ex vivo electroporation
・in vitro electroporation
・adhesive cells electroporation

CUY21EDITⅡ(in vivo&in vitro electroporator)

CUY21EDIT II is the true succesor of the best electroporator CUY21EDIT, which has a lot of great performance. CUY21EDIT II is easy to be used and generates the accurate pulses. As well as succeeding the great function of CUY21EDIT, BEX technology enable CUY21EDIT II to generate every pattern of the pulses for both of in vivo and in vitro. Moreover, CUY21EDIT II is the first electroporator to generate constant current pulses. CUY21EDIT II performs combination of constant voltage pulses and constant current voltage pulses, which leads to a whole new electroporation world !

Every pattern of pulses

CUY21EDITⅡ（in vivo&in vitro electroporator） generates decaying pulses as well as square pulses CUY21EDIT generates. Moreover, CUY21EDIT II generates dual pulses CUY21EX generates. That is, CUY21EDIT II also generates 1. Poration Pulses (Pp), which make very small holes in the surface of the cell, and 2. Driving Pulses (Pd), which move DNA, RNA, etc. into the inside of the cell. CUY21EDIT II changes the polarity of Pd in every way.

CUY21EDIT II can change the polarity of the square pulses alternately.

CUY21EDIT II generates dual pulses with square pulses and can change the decay rate of the voltage.

CUY21EDIT II can change the polarity of the decaying Driving Pulses in Dual pulse mode alternately. The convert of the capacitance of the condenser enable decay rate to be changed.

CUY21EDIT II can alse change the polarity of the square Driving Pulses in Dual pulse mode alternately. The decay rate can be changed.

5.7-inch touch panel for easy use

CUY21EDIT II is used by 5.7 inch touch panel. It is very easy to operate CUY21EDIT II visually by the touch panel. The visual operation avoids the mistakes of which parameter to be set.

Parameter setting display

Value setting display (numeric keypad shows up)

The first electroporator to generate constant current pulses

CUY21EDIT II is the first electroporator to generate constant current pulses. In electroporation, the key to success is current which is running between the sample. Previous electroporators, however, set only voltage value and can't set current running between the sample. BEX previously equipped all BEX electroporators with the function to measure the impedance, so that the user can calculate the current by the set voltage and measured impedance. Now, CUY21EDIT II initialy can set current values to release the user from the calculation of the current value by the voltage and the impedance.

Pulse data is exported to a USB memory.

CUY21EDIT II exports all data of generated pulses to a USB memory. It is not necessary to make a note for electroporation data. You can use digital data in your PC.

A lot of application

CUY21EDIT II generates every pattern of the pulses to fit every application of electroporation. As well as in vivo electroporation, CUY21EDIT II is suitable for electroporation for suspension cells of animals or plant cells and for electroporation with adhesive cells attached in the plate or dish. CUY21EDIT II is used for iPS cell research.

All data of executed voltage and current pulses are recorded.

CUY21EDIT II records all data of executed volatege and current. It is not necessary to make a note of values of each electroporation. This function makes the experiments more efficient. CUY21EDIT II displays the executed parameter and can generate the same parameter as the displayed parameter.

display_history

'History' display to track the parameter. CUY21EDIT II can generate this parameter in this display.

※The spec and appearance of the product(s) above can be changed for improvement without notice.

Applications

GONAD

GONAD: Genome editing via Oviductal Nucleic Acids Delivery
is a simple and powerful method to genome edit zygotes by electroporation of the oviduct with zygotes inside.

Easy 3 Steps！

GONAD-step1

STEP1: Take the oviduct from a mouse. Zygotes are inside the oviduct.

GONAD-step2

STEP2: Inject Cas9 to the oviduct.

GONAD-step3

STEP3: Grab the oviduct by the tweezer-like electrode of LF650P3 and electroporate it.

That's it！！

Result

２Result of 2-cell embryos after electroporation

GONAD-result

Advantage of Constant Current for GONAD

Constant current is the best choice for GONAD!

In electroporation,

Damages depend on how much current is running → Current is a key to success for electroporation

Ex: When the oviduct is grabbed by the tweezer-like electrode...

Even if voltage is constant, current value depends on the distance between the electrodes, the condition (oxidization) of the electrodes, etc.

In constant current mode (Square (mA) mode), CUY21EDIT II controls as current runs constantly.

That is, in GONAD with CUY21EDIT II and tweezer-like electrodes (LF650P series), constant current runs through the oviduct even if the strength to grab the oviduct is different in each trial.

The electrical parameter is fixed in in vivo electroporation, which has many parameters for optimization.

Zygote Electroporation (GEEP method)

Please visit here for GEEP

in vitro Electroporation (culture cell electroporation)

The below is the list of some of our performance for electroporation to the cultured cells by using CUY21EDIT II. Please ask us if your taget cell isn't in the list.

Human Tissue-Derived Cells
Name of cell	Tissue	JCRB No.	Viability	Efficiency
MG-63	Bone	IFO50108(JCRB), RCB1890	70%	87.50%
KG-1	Bone marrow	JCRB0065	75%	70%
THP-1	Peripheral blood	JCRB0112	50%	60%
Kasumi-1	Blood	JCRB1003	45%	65%
RAJI	Burkitt lymphoma	JCRB9012	60%	75%
MOLT-4	Peripheral blood	JCRB9031	82.50%	95%
LoVo	Colon	JCRB9083	60%	62.50%
HT-1080	Sarcoma	JCRB9113	80%	95%
Ramos(RA1)	Burkitt lymphoma	JCRB9119	70%	70%
CACO-2	Colon	RCB0988	70%	50%
HCT116	Colon	RCB2979	85%	90%
Saos-2	Bone	RCB3688	90%	87.20%
HaCaT	Skin	N/C	80%	75%
NCI-H1299	Lung	ATCC CRL-5803	85%	95%
U20S	Bone	ATCC HTB-96	80%	87%
A-172	Brain	JCRB0228	85%	90%
HCT-15=DLD-1	Colon	JCRB9094(DLD-1)	70%	80%
NUGC-3	Stomach	JCRB0822	75%	75%
PC-3	Prostate, Bone meta	JCRB9110	75%	92%
U937	Pleural effusion	JCRB9021	55%	60%
MDA-MB-231	Breast; Mammary gland	ATCC HTB-26	70%	75%
SK-BR-3	Breast; Mammary gland	ATCC HTB-30	80%	70%
293(HEK293)	Kidney	JCRB9068	76%	100%
293T	Kidney	RCB2202	100%	94%
A549	Lung	JCRB0076	87.5%	60%
HeLa	Cervix	JCRB9004	71%	58%
HL60	Peripheral blood	JCRB0085	80%	90%
HuH-7	Liver	JCRB0403	N/C	100%
Jurkat	Blood	JCRB0147	44%	85%
MCF-7	Pleural effusion	JCRB0134	78.8%	64%
NALM-6	Blood	RCB1933	34.1%	56%
PANC-1	Pancreas; Duct	RCB2095	96%	69%
HUV-EC-C	Umbilical cord, Vein	IFO50271(JCRB)	42.50%	82.40%
RPMI8226	Peripheral blood	JCRB0034	40%	67.50%
Daudi	Burkitt lymphoma	JCRB9071	70%	50%
TIG-1	Fetus lung	JCRB0501	75%	80%
A-431	Epidermis	JCRB0004	75%	63%
LNCaP	Prostate	ATCC CRL-1740	42%	100%
LK-2	Lung	JCRB0829	70%	71%
K562	Pleural effusion	JCRB0019	75%	100%
HSC-3	Tongue	JCRB0623	86.30%	7.90%
A2058	Melanoma	ATCC CRL-11147	85%	75%
Hep G2	Liver	JCRB1054	60%	60%
MRC-5	Fetus lung	JCRB9008	65%	75%
T24	Bladder	JCRB0711	67.50%	85%
Ca Ski	Uterine cervix	IFO50007	75%	55%
Mewo	Skin	JCRB0066	70%	80%
A375	Skin	EC88113005	70%	80%
PC9	Lung	EC90071810	60%	70%
SH-SY5Y	Bone marrow	EC94030304	60%	65%
Mouse Tissue-Derived Cells
Name of cell	Tissue	JCRB No.	Viability	Efficiency
P-815	Tumor	JCRB0078	50%	70%
B16-F10	Skin	RCB2630	55%	60%
LLC	Lung	JCRB1348(3LL)	70%	80%
MC3T3-E1	Newborn, Calvaria	RCB1126	75%	80%
Hepa 1-6	Liver	RCB1638	75%	85%
RAW264.7	Ascites	ATCC TIB-71	45%	45%
Colon-26	Rectum	RCB2657	80%	80%
NMuMG	Breast, Mammary gland	ATCC TIB-1636	60%	50%
MIN6b	Pancreas	N/C	87.50%	87.50%
Neuro-2a	Brain	IFO50081(JCRB)	46%	93%
L	Adipose, Areolar	JCRB9003	46%	93%
3T3 L1	Embryo	JCRB9014	51%	82%
NIH3T3	Embryo	JCRB0615	64%	100%
Colon 26 subline	Colon	Sakata et al. 1996	93.40%	91%
C2C12	Muscle	EC91031101	80%	75%
Rat Tissue-Derived Cells
Name of cell	Tissue	JCRB No.	Viability	Efficiency
C6	Brain	JCRB9096	-%	95%
Chinese Hamster Tissue-Derived Cells
Name of cell	Tissue	JCRB No.	Viability	Efficiency
CHO-K1	Ovary	JCRB9018	83%	100%
Others
Name of cell	Tissue	JCRB No.	Viability	Efficiency
COS-1	Kidney	JCRB9082	100%	100%
COS-7	Kidney	JCRB9127	69.10%	100%
MDCK(NBL-2)	Kidney	JCRB9029	90%	85%

Spec

Poration Pulse (Pp)

Voltage	1 - 400 V in 1 V resolution
Pulse length (Ppon)	0.01 - 99.9 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms
Pulse interval* (Ppoff)	0.01 - 99.9 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms
No of pulses	1

*Interval between poration and driving pulse

Driving Pulse (Pd)

Decay (V) mode (Decay pulse)
Voltage	1 - 350 V in 1 V resolution
Maximum current	10 A
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Condenser capacity	Choice from 3.3 uF, 10 uF, 33 uF, 100 uF, 330 uF and/or 940 uF Multiple choices are avialable, Min: 3.3 uF; Max: 1,416.3 uF
No of pulses	1 - 1,000 number(s); On-Off switchable

Driving pulse (Pd)

Square (V) mode (Constant-voltage square pulse)
Voltage	1 - 250 V in 1 V resolution
Maximum current	1 A (1,000 mA)
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval** (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 99% in 1% resolution.
No of pulses	1 - 1,000 number(s); On-Off switchable

** In case decay rate is not 0%, the minimum time is 0.1 ms.

Driving pulse (Pd)

Square (mA) mode (Constant-current square pulse)
Current	1 - 1,000 mA in 1 mA resolution
Maximum voltage	200 V
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval** (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 99% in 1% resolution.
No of pulses	1 - 1,000 number(s); On-Off switchable

** In case decay rate is not 0%, the minimum time is 0.1 ms.

Decay (V) + Square (V) mode

Plural poration pulse (PLPp)
Voltage	1 - 350 V in 1 V resolution
Maximum current	10 A
Pulse length (PLPpon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval (PLPpoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 50% in 10% resolution.
Condenser capacity	PLPp (+): 2,429 uF PLPp (+/-), PLPp (Alt): 1,416 uF
No of pulses	1 - 1,000 number(s); On-Off switchable

Driving poration pulse (Pd)
Voltage	1 - 200 V in 1 V resolution
Maximum current	1 A (1,000 mA)
Pulse length (Pdon)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Pulse interval (Pdoff)	0.05 - 1,000 ms Resolution: - 0.01 ms between 0.01 - 9.99 ms - 0.1 ms between 10.0 - 99.9 ms - 1 ms between 100 - 1,000 ms
Decay rate	0 - 99% in 1% resolution.
No of pulses	1 - 1,000 number(s); On-Off switchable

Miscellaneous

Impedance measurement	0 - 39KΩ Resolution: - 0.001KΩ between 0- 1KΩ - 0.01KΩ between 1 - 10KΩ - 0.1KΩ between 20 - 39KΩ
Voltage measurement	-512 - +512V in 1V resolution
Current measurement	-10.23A - +10.24A in 0.01A resolution (Pd is decaying) -1023mA - +10.24mA in 1mA resolution (Pd is square)
Memory	4GB (Over 20000 programs are recordable)
History record	The last 100 programs are saved. These program are exported to a PC by USB device. CSV format file(.csv) is output.

Other

Power	100 - 115 or 220 V 50/60 Hz
Fuse	10 A (6.3 mm X 20 mm)
Dimensions	W 240 mm X L 380 mm X H 190 mm (w/o rubber stand, umbo)
Weight	9 kg　240 mm(W) X 380 mm (D¹) X 190 mm(H²)

in vivo&in vitro electroporation Application

A lot of applications for electroporation to tissues, adhesive cells, etc. with CUY21 series by BEX (electroprator) and a lot of various unique electrodes.

Electrode for CUY21 series

Reference for CUY21 series (electroporation)

method	species	target
in vivo　electroporation （CUY21）	adult mouse	skin, musle, kidney, liver, spleen, blood tube, bladder, testis, ovary, brain, brown adipose tissue, zygote
	adult rat	muscle
	new born mouse and rat	brain, retina, cornea
	hamster	muscle
	rabbit	muscle, knee joint
	zebrafish	tail fin, retina
	Xenopus	embryo, retina (tadpole)
	cricket	embryo
	honey bee	brain
	Terrestrial slug (Limax valentianus)	Whole body
in utero electroporation （CUY21）	mouse embryo	brain, spinal cord, retina, internal ear
in utero electroporation （CUY21）	rat embryo	brain
ex utero electroporation （CUY21）	mouse embryo	brain
ex utero electroporation （CUY21）	mouse / rat embryo	whole embryo (brain slice, neural tube)
in ovo electroporation （CUY21）	chick embryo	neural tube, eye capsule, leg bud (mesoblast), body segment (mesoblast), plan heart area (mesoblast), posterior mesoblast, plan trache-bronchi area, blastodermic layers
in ovo electroporation （CUY21）	uzura embryo	neural tube
ex ovo electroporation （CUY21）	chick early embryo	New’s culture
ex ovo electroporation （CUY21）	chick embryo	liver, cerebellum
ex vivo electroporation （CUY21）	mouse	brain, retina, small intestine, tooth germ, internal ear
	rat	brain
	chick embryo	spinal cord, brain stem, retina, anterior stomach
in vitro electroporation （CUY21）	adhensive
in vitro electroporation （CUY21）	primary cells , cell lines, etc.

Maintenance support for electroporators, cell fusion units, electrodes.

We welcome the request of maintenance support for electroporators (CUY21 series: CUY21EDIT II, CUY21EDIT, CUY21Vivo-SQ and CUY21Vitro-EX), cell fusion units (LF101 and LF301), electrodes, other accessories. Our engineers will be in direct charge of the products. Please ask us.